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      Cobra Biologics

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      Entrevista para GMP Operator

      8 de jul. de 2019
      Candidato(a) sigiloso(a) à entrevista
      Keele, England
      Nenhuma oferta
      Experiência positiva
      Entrevista difícil

      Candidatura

      Candidatei-me online. O processo levou 4 semanas. Fiz uma entrevista na empresa Cobra Biologics (Keele, England).

      Entrevista

      I interviewed for a GMP operator. Interview was split between 4 sections: 1. with HR 2. Arithmetic based test (30 mins) 3. GMP Production manager along with 2 Senior scientists 4. Head of Production. Interview lasted about 2hr 55 mins and this was followed by a 15-20 mins tour of their clean-room production facility (Downstream processing, specifically). The interview was intense with many subject based questions around cell culture, GMP, microbial fermentation, and down stream processes including plasmid purification. HR interview section is mainly get you to calm your nerves down and get in the interview mood. GMP manager and Senior scientists are there to test your competence and specific skills and experiences around the role; they put you under pressure to see how you could endure the seemingly never ending questions. Head of production was like a psycho analyst who want to know your commitment, knowledge around industry and possible long term goals. Tour guides are fun and help you learn more about the kind of work they do there and how they find working at the place; don't ask too specific questions and the products they produce or the cell lines they work with because they'll tell you that sensitive company information. All interviewers were friendly and showed interest in the process, but also with a commitment to absolutely test your competence around the role.

      Perguntas de entrevista [8]

      Pergunta 1

      Explain what GMP is why it's important. What is the role of a GMP operator?
      Responder à pergunta

      Pergunta 2

      Calculate cell viability % and plasmid yield. What would you use to stain cells (not bacteria)? If you go for 'trypan blue' as I did, then explain the principle behind the stain. What would you use to preserve/bank cells? Hint cryoprotectants.
      Responder à pergunta

      Pergunta 3

      Draw and annotate bacterial or cell growth curve? Why do cells enter the stationery growth phase on the curve you have drawn?
      Responder à pergunta

      Pergunta 4

      What's the generation time of E. coli and what's its optimum growth temperature?
      Responder à pergunta

      Pergunta 5

      What are the conditions for microbial fermentation?
      Responder à pergunta

      Pergunta 6

      If you've done transformation/transfection before what method did you use. Cobra uses electroporation, which you may only be told after you tell them yours :). Do you have plasmid purification experience? If yes how did you carry it out-if you used a kit such as Qiagen's Plasmid purification Kit you may be asked to name the manufacturer
      Responder à pergunta

      Pergunta 7

      You will be given ~5 pictures of pressure meters and asked to record the readings on the meters. Then you will calculate the differences in pressure (not as complex as you may think, it's just subtracting one reading from the other and they'll give you a calculator). There will also be reference ranges for each meter and you have to say whether the meter reading is within (pass) or outwith (fail) the reference values.
      Responder à pergunta

      Pergunta 8

      You'll be provided with a short text describing how to correctly write data in a GMP format. Then you'll be provided with an excerpt from a GMP data book and asked to identify any wrong entries.
      Responder à pergunta
      5